Mandibular (Facial Vein/Artery) Sampling

This technique is currently employed or limited to adult mice and rats. This technique is preferred over other techniques, due the following reasons:

• Medium to large blood volumes can be obtained.
• Repeated sampling is possible by alternating sides of the face.
• Requires less hands-on training and is safer than retro-orbital bleeding.
• Doesn’t require anesthesia.
• Excessive bleeding can be avoided or controlled by using smaller needle.

Sample Quality

• Variable - mixed (arterial/venous).
• Contamination due to hair or skin possible.

Methodology

• Good restraint is essential for successful  blood collection.
• Check the Medipoint website for clear description and anatomical location.
• Apply gentle pressure for 20-30 seconds after collection to ensure hemostasis.

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References

MEDIpoint, Inc.

NIH guidelines for Survival Bleeding of Mice and Rats.